Can the smart paper be the observation from the excel spreedsheet? Things to thi

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Can the smart paper be the observation from the excel spreedsheet?
Things to think about
Processing 1D data:
a) Background
• What information does a 1D proton spectrum provide?
• What are the overall processing steps of 1D-NMR data?
• What is Fourier Transformation?
• What is phasing and how to phase?
• What is chemical shift?
b) How to:
• Start Topspin and open up a file.
• Execute Fourier Transformation.
• Zoom in and out and manipulate the viewing of the spectrum.
• Phase manually or automatically.
• Correct the baseline of the spectrum.
• Calibrate the chemical shift axis.
• Pick peaks.
• Integrate peaks.
• Start Plot-editor for making nice spectral plots.
c) Parameter to be investigated: LB
Procedures to be evaluated: Fourier Transform, Phasing, Integration.
d) Samples: Spectra from nail-polish remover analysis experiment in CHM 303
Obtaining your first 1H-spectrum and understanding Fourier Transformation:
a) Background:
• What is a spin and what is magnetization?
• What is the “rotating frame”?
• What is an rf-pulse?
• What is a Free Induction Decay?
• What is exponential window multiplication?
• How is a received analog signal digitized?
• What is the Nyquist criterion?
b) How to
• Start a new experiment.
• Begin the data acquisition.
c) Commands to be investigated: ZG, RGA
d) Samples: D2O or DCCl3 with H impurity. Use of Excel Demo file
Exploring settings for obtaining a 1H-spectrum and first simulation of NMR spectra
a) Background
• What is the meaning of: spectral window, carrier frequency, off-set, acquisition time, dwell time?
• What is dynamic range and receiver gain?
• What is signal-to-noise?
b) How to
• Change experimental parameters
• Start and use the NMR simulator software.
• Switch nuclei, experiments and samples in NMR simulator.
c) Parameters to be investigated: AQ, TD/SI, SW, NS, RG, O1
d) Samples: D2O or DCCl3 with H impurity.
The instrument basic procedures
a) Background:
• What are the major components of the instrument?
• What affects the resolution of the spectrum?
– Effect of poor shimming on line shapes
– Spinning a sample
– Spinning side-bands
• What does tuning and matching mean?
• What does “lock” mean and what does it do?
b) How to
• Inject a sample.
• Spin a sample.
• Lock a sample.
• Tune and match.
• Shim a sample.
• Watch in real time changes on a spectrum in GS mode.
a) Parameters to be investigated: AQ, TD/SI, SW, SF, NS, RG, O1:
d) Samples: D2O or DCCl3 with H impurity.
Spin-spin coupling, switching between proton and carbon
a) Background:
• What is spin-spin coupling?
• How to read the symbol nJXX?
• What are first and second order spectra?
• What is homonuclear and heteronuclear coupling?
• What is broad band decoupling?
• Considerations when switching to a different pulse program possibly involving different nuclei.
b) How to
• How to read in parameter files
• Obtain a 13C spectrum with or without broad band decoupling.
• Analyze multiplets using Topspin.
c) Procedures to be evaluated:
• Comparison of coupled and decoupled 13C spectra.
d) Samples: 3,3 dimethylpentane and other n-heptane isomers in CDCl3.
Comments from Customer
I need a journal entry


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